AN EXTENSIVE REVIEW ON PRASUGREL

In an attempt prasugrel increase in the overall use of this medication comparing to the anti thembolytics like heparin and wararin, with less patients complaints includes side effects a significant portion of patients receiving the medication for off label indications. More importantly, this agent is used in a substantial number of patients with known contraindications. Prasugrel are more intense platelet inhibition confirmed by the observation of highest percentage of minor or moderate bleeding among patients taking prasugrel, although there was no significant increase in the rate of severe, major, or life-threatening bleeding. Keywords: prasugrel, thembolytics, wararin, hepari

ROLE OF CLINICAL PHARMACIST IN ASSESSMENT OF DRUG RELATED PROBLEMS OF CARDIOVASCULAR AGENTS IN DEPARTMENT OF CARDIOLOGY IN A TERTIARY CARE HOSPITAL – A PROSPECTIVE OBSERVATIONAL STUDY

In total of 173 prescriptions of the patient, the total number of 329 drug interactions (DIs) was found, in which the 240 interactions were found in male and 89 in the female bearing 72.90% and 27.10%, respectively. Patient was of various age groups, in which the maximum frequency was seen in the age group of 61–70 years having 105 interactions bearing the percentage of 31.9%. According to the participation of different age group of the male and female with their corresponding age. The participation of male is high having frequency of 240 (72.9%) than female participation of frequency 89 (27.1%). The participation of male in the age group of 61–70 years is 83 and the female is 40 in the age group of 51–60 years. Potential DIs (PDIs) were categorized based on the gender. In that compared to 13 (40.6) females, males 19 (59.4%) were found to have more PDIs. Our study is more PDIs in adult patients. Because in adults lacking of nutrition’s and in elderly patients multiple prescribers, multiple drugs and multiple diseases. The number of potential drug-DI (PDDI) increased with an increase in the number of drugs prescribed. The numbers of drug prescribed increase with age. This DI has a potential to increase or decrease the therapeutic effect or to increase the risk of adverse drug reaction. An increased awareness of PDDIs, rational coprescription of drugs, and a close monitoring of patients in whom these drugs are prescribed are recommended. The recommendation is based on the special monitoring and the perspiration of the clinical pharmacist. The DI observed in the geriatric patient is more severe and common in compared to the other groups of study. The geriatric patient is physiological disability in correspond with the first-pass metabolism and the presence of the other diseases which also enables the multiple prescriptions causing polypharmacy. The gender specification also the cause of the interaction, the female is more prone to the DI due to the hormonal distribution in the body and inability of the physiological function to absorb and the distribution. The special training should be provided to the pharmacist for looking forward of the geriatric patient and female patient. The training regarding the prescription their adherence, use, toxicity, and dosage regimen is being properly enabled in the training for the practical application. This study helps to know the different interaction related to the cardiovascular agent with own class of the drug and the other class of drugs used therapeutically to care the disease

Roadmap for Optical Tweezers 2023

Optical tweezers are tools made of light that enable contactless pushing, trapping, and manipulation of objects ranging from atoms to space light sails. Since the pioneering work by Arthur Ashkin in the 1970s, optical tweezers have evolved into sophisticated instruments and have been employed in a broad range of applications in life sciences, physics, and engineering. These include accurate force and torque measurement at the femtonewton level, microrheology of complex fluids, single micro- and nanoparticle spectroscopy, single-cell analysis, and statistical-physics experiments. This roadmap provides insights into current investigations involving optical forces and optical tweezers from their theoretical foundations to designs and setups. It also offers perspectives for applications to a wide range of research fields, from biophysics to space exploration

Allosteric Transition Induced by Mg2+ Ion in a Transactivator Monitored by SERS

We demonstrate the utility of the surface-enhanced Raman spectroscopy (SERS) to monitor conformational transitions in protein upon ligand binding. The changes in protein's secondary and tertiary structures were monitored using amide and aliphatic/aromatic side chain vibrations. Changes in these bands are suggestive of the stabilization of the secondary and tertiary structure of transcription activator protein C in the presence of Mg2+ ion, whereas the spectral fingerprint remained unaltered in the case of a mutant protein, defective in Mg2+ binding. The importance of the acidic residues in Mg2+ binding, which triggers an overall allosteric transition in the protein, is visualized in the molecular model. The present study thus opens up avenues toward the application of SERS as a potential tool for gaining structural insights into the changes occurring during conformational transitions in proteins

Analysis of protein acetyltransferase structure–function relation by Surface Enhanced Raman Scattering (SERS): A tool to screen and characterize small molecule modulators

Among the different Posttranslational Modifications (PTMs) that significantly regulate the protein function, lysine acetylation has become the major focus, especially to understand the epigenetic role of the acetyltransferases, in cellular physiology. Furthermore, dysfunction of these acetyltransferases is well documented under pathophysiological conditions. Therefore, it is important to understand the dynamic structure–function relationship of acetyltransferases in a relatively less complicated and faster method, which could be efficiently exploited to design and synthesis of small molecule modulators (activators/inhibitors) of these enzymes for in vivo functional analysis and therapeutic purposes. We have developed Surface Enhanced Raman Scattering (SERS) method, for acetyltransferases towards this goal. By employing SERS, we have not only demonstrated the autoacetylation induced structural changes of p300 enzyme but also could use this technique to characterize and design potent, specific inhibitors as well as activators of the p300. In this chapter we shall describe the methods in detail which could be highly useful for other classes of HATs and PTM enzymes